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. 2019 Oct 30;10:1362. doi: 10.3389/fpls.2019.01362

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Copyright © 2019 He, Zhang, Zhao, Qi, Kang and Guo

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Molecular analysis of transgenic B. distachyon. (A) Integration of Fg00677-RNAi construct in transgenic plants analyzed by PCR amplification using Fg00677 and Bar gene-specific primers. (B) Integration of Fg08731-RNAi construct in transgenic plants analyzed by PCR amplification using Fg08731 and Bar gene-specific primers. (C) Integration of CYP51-RNAi construct in transgenic plants analyzed by PCR amplification using CYP51BAC and Bar gene-specific primers. The genomic DNA from non-transformed control (NTC) plants was used as negative control. The plasmid DNA, including FG00677-RNAi construct, FG08731-RNAi construct, or CYP51-RNAi construct was used as positive control (PC); M indicates DL2000 DNA marker.