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. 2019 Nov 5;9:16039. doi: 10.1038/s41598-019-52366-4

Figure 2.

Figure 2

VSSC Sensitivity. (A) The detection of 60–296 nm PS particles by VSSC. The CytoFLEX can detect as small as 60 nm PS nanoparticles, and can resolve as small as 70 nm. (B) The detection of 98.6–293 nm Si particles by VSSC. 98.6 nm Si particles with a RI of 1.44 can be fully resolved by VSSC. (C) VSSC detection of viruses. Unlabeled 95 nm HAdV-5, 100 nm HIV-1, and 110 nm MLV can be fully resolved by light scatter on the CytoFLEX. (D) A contour plot prepared using Mie-theory RI curves scaled to the CytoFLEX VSSC intensities. The data from (A–C) were overlaid on the plot to verify the accuracy of the scaling. All samples were collected in triplicate and these data represent the population means. VSSC gain = 400; VSSC-H threshold = 3000. The VSSC-H threshold for HSV-1 and Vaccinia was 40 K and 100 K, respectively.