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. 2019 Nov 5;9:16039. doi: 10.1038/s41598-019-52366-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sizing Viruses by Flow Cytometry. (A–D) VSSC-H population statistics for (A) HAdV-5, (B) HIV-1, (C) MLV, and (D) HSV-1. Each virus was read in triplicate and the data represent the average. (E) Fitting a VSSC-H Intensity vs. Size curve for RI 1.47 at 405 nm in order to calculate virus sizes. (F) The calculated size characteristics for each virus in (A–D). In the chart above, the calculated peak diameter is displayed along with the size range. The mean and median sizes are also included in the table below, ±the SEM.