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. 2019 Nov 5;9:16006. doi: 10.1038/s41598-019-52516-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression pattern of cardiac mesoderm, definitive endoderm, and neural ectoderm genes in adherently expanded CMCs. Quantitative RT-PCR analysis showed upregulation of cardiac mesoderm and downregulation of definitive endoderm and neural ectoderm genes in CMCs derived from RH5 (a), RH6 (b), and iPS (c) cell lines. Analysis was performed at passage 4 (P4) for (b) and (c). All data: mean ± SD. The statistical differences between P4 and P10 in (a) were analyzed by the unpaired t-test.