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. 2019 Nov 5;9:16006. doi: 10.1038/s41598-019-52516-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vitro differentiation potential of adherently expanded CMCs in ABC medium. (a) Schematic illustration of induction protocols for differentiation of passage 10 CMCs into cardiomyocytes, endothelial, and smooth muscle cells. D: Day; SB43: SB431542; Pur: Purmorphamine; -Ins: Minus insulin; -Vit. A: Minus vitamin A. (b) Morphology of induced-CMCs into cardiomyocytes, endothelial and smooth muscle cells. Scale bars: 200 µm. (c) Expression analyses of cardiomyocytes (cTNT, MLC2v, and GJA1), endothelial (KDR and vWF), and smooth muscle (PDGFRa and MYH11) cell specific genes compared to undifferentiated CMCs. All data: mean ± SD. (d) Immunofluorescence staining of cTNT, vWF, and α-SMA. Cells were counter-stained with DAPI. Scale bars: 100 µm.