Table 1.

Description of the different isolation and expansion conditions for Donor I.

DONOR I
Abbreviation	Description
Direct(AB + FGFlow)	Bone marrow was directly seeded onto flasks with α-MEM +10% AB serum +1% P/S and 5 ng/mL FGF2 (all from R&D)
ACK(AB + FGFlow)	Prepared with Ammonium-Chloride-Potassium (ACK) lysing buffer (Gibco) and cells were seeded with α-MEM +10% AB serum +1% P/S and 5 ng/mL FGF-2
Lympho(AB + FGFlow)	Bone marrow was centrifuged in a density gradient medium Lymphoprep (Stemcell Technologies) to isolate mononuclear cells before culturing in α-MEM +10% AB serum +1% P/S and 5 ng/mL FGF2 (all from R&D)
Direct(PL)	Bone marrow was directly seeded onto flasks with α-MEM +1% P/S (Gibco) +10% PL + heparin (2I/U per mL)

Abbreviations: α-MEM, alpha minimum essential medium; AB serum, human serum from type AB donors; P/S, penicillin/streptomycin, FGF2, fibroblast growth factor 2.