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. 2019 Oct 30;10:1312. doi: 10.3389/fpls.2019.01312

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Copyright © 2019 Thirkell, Cameron and Hodge

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Excess ¹⁵N content in Maris Otter and Meridian shoots (calculated by subtracting shoot ¹⁵N content in each “Access” unit from the mean of the corresponding values in the “No Access” units). Shoot ¹⁵N enrichment was significantly higher than “no access” controls when supplied as nitrate to Meridian barley in High-N plots. Circles represent individual data points, boxplot centre bars represent the median values. High-N + ammonium groups are represented by white bars, High-N + nitrate by light blue bars, Low-N + ammonium by dark grey bars and Low-N + nitrate by dark blue bars. Data shown are means ± SEM, n = 3. Bars sharing the same letter are not significantly different.