FIGURE 2.

Multiple sequence alignment of Chi23 and other chitinases from the GH18 subfamily B. Using ESPript 3.0, secondary structures of Chi23 are shown above alignment and those of the endochitinase PPL2 (PDB code 2GSJ) from Parkia platycephala seeds under alignment. Helices are indicated by springs, strands by arrows, turns by TT letters. Identical residues are shown in white on a black background, and similar residues are in bold black. Chitinases from bacteria and eukaryotes are marked by black and green squares, respectively. The conserved DxDxE motif containing the catalytic Glu (marked by a star) is boxed. Selected residues of Chi23 for mutation are indicated by red circles.