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. 2019 Oct 30;10:2457. doi: 10.3389/fmicb.2019.02457

FIGURE 4.

FIGURE 4

Hydrolysis of crystalline chitin, colloidal chitin and chitooligomers by the purified Chi23. (A) TLC showing the complete hydrolysis of crystalline chitin (powder) and colloidal chitin by Chi23. Lane Std, standard mix of (GlcNAc)1–6; lane P + E, hydrolysis of crystalline chitin; lane C + E, hydrolysis of colloidal chitin. (B) TLC showing the complete hydrolysis of chitooligomers (GlcNAc)2–6 by Chi23. Lane Std, standard mix of (GlcNAc)1–6; lanes 2 + E to 6 + E, hydrolysis of chitooligomers from (GlcNAc)2 to (GlcNAc)6, respectively. (C) Time course of (GlcNAc)6 hydrolysis by Chi23. Hydrolysis of (GlcNAc)6 was carried out at pH 5.0 and 60°C for 5–120 min and the concentrations of the enzyme and substrate used were 32.8 nM and 6.2 mg/mL, respectively.