TABLE 1.

Primers used in this study.

Gene product	Primer	Sequence (5′-3′)a
Chi23	Chi23-F Chi23-R	AAGAAGGAGATATACATATGTCTAAAACCATTACCTATTATAACTCG (NdeI) TGGTGGTGGTGGTGCTCGAGGCTATTCAGCGATTGTGCAA (XhoI)
N9A	N9A-F N9A-R	TATTATGCCTCGGGCGCGGTCCCGCTCAT CCCGAGGCATAATAGGTAATGGTTTTAGACAT
G79A	G79A-F G79A-R	TCATTTGGTGGCGCCACCATGGGTTCCAACGCTT TGGCGCCACCAAATGAGATCAATACTTTCTGCCCCTTGTG
T80A	T80A-F T80A-R	CGCCATGGGTTCCAACGCTTATCGTTCGTTGTCA TTGGAACCCATGGCGCCGCCACCAAATGAGATC
D115A	D115A-F D115A-R	ATATAGCTTATGAAGATACGGCCGCGTTCACTGGTCAAG GCCGTATCTTCATAAGCTATATCCACGCCATCTAACTGAT
E117A	E117A-F E117A-R	TATAGATTATGCAGATACGGCCGCGTTCACTGGTCAAG GCCGTATCTGCATAATCTATATCCACGCCATCTAACTGAT
Q157A	Q157A-F Q157A-R	ATTTCTCATGCACCTGCACCTCCTTATTTGGAGCAAGGC TGCAGGTGCATGAGAAATGATGTAGTCAGGGCTCGG
Y189A	Y189A-F Y189A-R	TTAAATGTGCAGTTTGCCAACAACCCGCCATGG GGCAAACTGCACATTTAACCAGTCAATCTCTTGTCCC
N190A	N190A-F N190A-R	TGTGCAGTTTTACGCCAACCCGCCATGGAGTGCT TTGGCGTAAAACTGCACATTTAACCAGTCAATCTCTTGTCCCA
D229A	D229A-F D229A-R	TGTCACGCAGAACGCTGCGGGTTCTGGGTATATGC AGCGTTCTGCGTGACAGGAAAGCCAGCAATAACCTTCTC
W260A	W260A-F W260A-R	ATTATGAATGCGCAGTTCTCAAGTGACCACAATGGT AACTGCGCATTCATAATGCCGCCAAGGCTAGAT
Q261A	Q261A-F Q261A-R	ATTATGAATTGGGCGTTCTCAAGTGACCACAATGGTGATT AGAACGCCCAATTCATAATGCCGCCAAGGCTAGAT

^aThe cloning sites used are underlined, and the restriction enzymes are indicated in parentheses.