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. 2019 Nov 5;9:16005. doi: 10.1038/s41598-019-52505-x

Figure 1.

Figure 1

BPA and BPS disrupt acini organization of MCF-12A. MCF-12A cells were grown as acini in Matrigel. 5,000 cells per well were seeded in 8-well chamber slides as described in Material and Methods. Cells were treated every 4 days with EtOH (negative control), 1 nM E2 (positive control), or the indicated concentration of BPA and BPS and allowed to grow for 8 (A), 16 (B) and 25 days (C). Spheroids were immunostained with antibody against integrin-6 (green) to stain the basement membrane and counterstained with DAPI to visualize cell nuclei (blue). Confocal images are representative of three independent experiments, bars represent 10 µm.