Figure 2.

BPA and BPS increase MCF-12A acini volume. MCF-12A cells were grown as acini in Matrigel. 5,000 cells per well were seeded in 8-well chamber slides as described in Material and Methods. Cells were treated every 4 days with EtOH (negative control), 1 nM E2 (positive control), or the indicated concentration of BPA and BPS and allowed to grow for 8, 16 and 25 days. The volume of the spheroids was measured in μm³ at day 8 (A), day 16 (B) and day 25 (C). Results from 3 separate experiments, assessing at least twenty acini per condition, are graphically represented as mean volume treatment/mean volume control ± S.E.M. Statistical significance for BPA and BPS treatments was assessed by two-way ANOVA with Tukey’s post-hoc tests *denotes p < 0.05 and **denotes p < 0.01 compared to solvent control. A paired t-test was performed for the E2 condition. Acini smaller than 25 μm in diameter were excluded from the analysis, as they represent cells that did not form structures.