Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 5;9:16005. doi: 10.1038/s41598-019-52505-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

BPA and BPS treatments inhibit lumen formation of MCF-12A cells in Matrigel. Percentage (%) of acini with a lumen at day 16 (A) and at day 25 (B) was determined by analyzing confocal images. Cells were treated as described in Material and Methods with indicated concentration of BPA, BPS and E2. Structures with more than six cells present in the centre of the lumen were scored as structures with no lumen. Data corresponds to mean ± S.E.M and the results from three independent experiments, where a minimum of 20 acini were analyzed per experiment. (*p < 0.05), (**p < 0.01) and (***p < 0.001) indicate significant differences between treatments and negative control. Acini smaller than 25 μm in diameter were excluded from the analysis, as they represent cells that did not form structures.