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. 2019 Nov 5;9:16005. doi: 10.1038/s41598-019-52505-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Proliferation of MCF-12A cells treated with BPA, BPS and E2. MCF-12A cells were seeded at 20,000 cells per well in a 24-well plate in MCF-12A 5% charcoal stripped horse serum, 0.5 μg/mL hydrocortisone, 100 ng/mL cholera toxin, 10 μg/mL insulin, 1X pen/strep and phenol red free DMEM/F12 media and treated with the indicated chemicals every two days. Cells were counted after seven days of being treated with indicated concentration of BPA, BPS and E2 (A) or the indicated concentrations of EGF (B). Data corresponds to mean ± S.E.M from three independent experiments done in triplicates.