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. 2019 Nov 5;9:16059. doi: 10.1038/s41598-019-52558-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Notch signaling is activated during valve regeneration. (A) Confocal z-stack of a Tg(TP1:VenusPEST)/hspGFFDMC73A: UAS-E1b:NfsB-mCherry double transgenic embryo at 5 dpf. Tg(TP1:VenusPEST) signal of activated Notch signaling is restricted to the valves. Scale bar: 100 microns. (B) 1 day post MTZ treatments. Expansion of activation of Notch signaling throughout the ventricle is observed (blue arrows) Scale bar: 50microns. Confocal z-stacks of untreated Tg(hspGFFDMC73A: UAS-E1b:NfsB-mCherry) (C) and Tg(GSAIzGFFD703A: UAS-E1b:NfsB-mCherry) (F) double transgenic embryos at 6 dpf. Confocal z-stack of MTZ treated Tg(hspGFFDMC73A: UAS-E1b:NfsB-mCherry) (D), and Tg(GSAIzGFFD703A: UAS-E1b:NfsB-mCherry) (G), at 6 dpf. Confocal z-stack of MTZ treated and then during regeneration 48 hours DAPT treated Tg(hspGFFDMC73A: UAS-E1b:NfsB-mCherry) (E), and Tg(GSAIzGFFD703A: UAS-E1b:NfsB-mCherry) (H), at 6 dpf. (I) Each experiment was carried out at least 3 independent times with n = 10 embryos per experimental group. Scale bars: 20 micron.