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. 2019 Oct 30;10:1367. doi: 10.3389/fpls.2019.01367

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Copyright © 2019 Li, Bai, Pei, Yang, Mao, Zhang, Liu and Liang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SmGRAS1 binds to the SmKSL1 promoter and activates its expression. (A) Yeast one-hybrid (Y1H) assays shows the interactions between SmGRAS1/2 and the SmKSL1 promoter. SmKSL1 promoter + pGADT7 as the negative control and SmKSL1 promoter + pGADT7-SmERF6 as the positive control. (B) Dual-luciferase (Dual-LUC) assay shows the effects of SmGRAS1 on SmKSL1 promoter activation. (C) Electrophoretic mobility shift assay (EMSA) analysis of SmGRAS1 binding to the GARE-motif of the SmKSL1 promoter. Schematic diagram showing the GARE motif in the SmKSL1 promoter.