Figure 2.

Immunogenicity of the different vaccination regimens in rabbits. (A) Immunization schematic indicating the timing of immunizations and bleeds. Time points in brackets indicate blood draws which were taken for the group immunized with the SEC-purified protein only. (B) Serum binding antibodies elicited over the course of the immunization regimen to the matched antigen, purified from mammalian cells, that was used to immunize animals. The levels of serum binding antibody titres elicited by the different vaccination regimens of CAP256 SU Env were compared after the 3^rd (C) and 4^th (D) immunizations. Similarly, autologous V1V2 binding antibodies were quantified after the 3^rd (E) and 4^th (F) inoculation. Statistical comparisons between groups were performed using a two-tailed, unpaired Mann-Whitney test (*p < 0.05). The levels of binding antibodies are indicated as a fold-dilution derived from the fitted four-point linear regression curve using a threshold of the minimum + standard error of the minimum for each time point.