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. 2019 Oct 30;10:1350. doi: 10.3389/fphys.2019.01350

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Copyright © 2019 Lucas, Ao-ieong, Williams, Jani, Muller, Yalcin and Cabrales.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Heart staining for hypoxia with pimonidazole. Positive pimonidazole staining represents areas of hypoxia in the cardiac tissue. Amount of hypoxic areas increased with the proximity to the heart apex.