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. 2019 Oct 30;10:1292. doi: 10.3389/fphar.2019.01292

Figure 3.

Figure 3

Rapamycin reduces NLRP3 inflammasome activation by inhibiting NF-κB activation. (A, B) The expression of p-P65/P65 and IκBα assessed by western blot after high glucose treatment by time course. (C, D) P65 phosphorylation and IκBα analyzed by western blot after rapamycin treatment. (E–H) Western blot indicated that knockdown of P65 significantly reduced the expression of NLRP3, Caspase-1 and ASC protein. (I–L) Western blot indicated that treatment with NF-κB inhibitors significantly reduced the expression of NLRP3, Caspase-1 and ASC protein. (M) Translocation of NF-κB p65 was observed after high glucose treatment for 12h under a fluorescence microscope. *p 0.05 versus NC group; **p 0.05 versus HG or HG (control) group. Data are expressed as mean ± SD, n = 3. (N) Real-time PCR indicated that Knocking down p65 significantly reduced the mRNA expression of p65. *p 0.05 versus siRNA NC group. Data are expressed as mean ± SD, n = 3.