Fig. 1.

Effect of PTE on the anticonvulsant activity of CBZ (a–c) and TGB (d–f) in the iv PTZ seizure threshold test in mice. TGB was administered 15 min, while CZP and PTE were injected 30 min before the iv PTZ test. All the drugs were administered ip at a constant volume of 10 ml per kg body weight. Animals in the negative control groups received vehicle (5% Tween 80). Each experimental group consisted of 9–13 animals. Data are presented as the mean threshold dose of PTZ (in mg/kg body weight) + SEM and were analyzed using one-way ANOVA (CZP first myoclonic twitch F(4,54) = 42.60, p < 0.0001; generalized clonic seizures F(4,48) = 16.99, p < 0.0001; forelimb tonic extension F(4,48) = 11.02, p < 0.0001; TGB: first myoclonic twitch F(4,50) = 15.13, p < 0.0001; generalized clonic seizures F(4,52) = 13.49, p < 0.0001; forelimb tonic extension F(4,47) = 12.05, p < 0.0001) with the Tukey post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001 vs. the negative control (5% Tween-treated) group; #p < 0.05, ##p < 0.01, ###p < 0.001 vs. CZP-treated group. CZP, clonazepam; PTE, pterostilbene; PTZ, pentetrazole, TGB, tiagabine