Skip to main content
. 2019 Nov 5;10(6):e02324-19. doi: 10.1128/mBio.02324-19

FIG 4.

FIG 4

B. thetaiotaomicron’s fructan PUL is downregulated in WT mice relative to that in Rag1−/− mice. (A) Experimental design for in vivo experiment. (B) Average expression of fructan PUL in the ceca of WT mice relative to that in Rag1−/− mice at week 6 postcolonization. ***, P < 0.0001. (C) Time course of fructan PUL gene expression in WT and Rag1−/− mice feces. Normalized data are shown relative to PUL expression at day 7 in Rag1−/− mice. qRT-PCR analyses to measure expression of the fructan PUL in MM-levan (D) and MM-fructose (E) in the presence of SI culture supernatant from B. thetaiotaomicron-monocolonized WT or Rag1−/− mice (week 6). Expression is relative to that in MM-glucose. *, P < 0.01; **, P < 0.001. (F) Fold change in the expression of the fructan PUL in MM-fructose (relative to that in MM-G) under IgA− and IgA+ conditions. B. thetaiotaomicron-specific IgA (Bt IgA), test; human E. coli-specific IgA, nonspecific IgA control (n = 3 biological replicates for each experiment).