Figure 4.

Spinal Cord Neurons Express Multiple Neurotransmitter Phenotypes

(A) Whole-mount double immunolabeling between glutamate, GABA, glycine, ChAT, and serotonin. In black and white are single channel images of the merged images. Arrows indicate the double-labeled neurons.

(B and C) Soma sizes and spatial distribution of the detected double-stained neurons in the adult zebrafish spinal cord hemisegment.

(D–H) Quantification of percentage of glutamatergic, GABAergic, glycinergic, cholinergic (ChAT⁺), and serotonergic neurons that co-express other neurotransmitters.

(I) Schematic relationship of the neurotransmitters co-expression from the adult zebrafish spinal cord neurons.

Data are presented as mean ± SEM. For related data and antibodies information, see also Figures S2 and S3 and Table S1.