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. 2019 Jul 20;26:101280. doi: 10.1016/j.redox.2019.101280

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© 2019 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 4 — Protein expression levels and redox states of roGFP2 fusion reporters in SHuffle and wild type cells. Cells expressing either roGFP2-Orp1 (A) or Grx1-roGFP2 (B) were analyzed in a Western blot using anti-His antibodies. Redox states of the fusions were chemically interrogated using AMS. Samples treated with DTT served to identify the migration of the reduced (red) protein band while those not treated with AMS served to identify the oxidized band (ox), which are indicated with arrows. Wild type (MB5994) and SHuffle cells (MB5995) harboring empty vectors are shown.