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Change in oxidation state of the roGFP2 probes in cells during growth. Wild type (filled circles) or SHuffle cells (open circles) expressing either Grx1-roGFP2 (A and C) or roGFP2-Orp1 (B and D) were monitored for their growth at OD600nm (A and B) and the ratiometric response of the probes (OxD) was calculated based on the 400/488 nm excitation ratio, with emission measured at 510 nm (C and D).
