Fig. 7.

Response of redox probes to H₂O₂in wild type cells. Response to increasing concentrations of H₂O₂ of Grx1-roGFP2 (A and C) and roGFP2-Orp1(B and D) in wt cells (C2566) where measured using the CLARIOstar microplate reader (BMG Labtech). Injection of H₂O₂ was performed into microplate wells containing E. coli biosensor cells of an OD₅₀₀ of 2. Samples for fully reduced and oxidized controls were treated for 10 min with 10 mM DTT and 5 mM diamide, respectively. The OxD was calculated using the 405/488 excitation ratio with emission at 510 nm.