Table 1.
Bacterial strains and plasmids utilized in this study.
| Strains | Relevant Genotype | Source |
|---|---|---|
| NEB express T7 | E. coli BL21 fhuA2 lacZ::T7 gene1 [lon] ompT gal sulA11 R(mcr-73::miniTn10--TetS)2 [dcm] R(zgb-210::Tn10--TetS) endA1 Δ(mcrC-mrr)114::IS10 | NEB cat# C2566 |
| SHuffle express T7 | E. coli BL21 fhuA2 lacZ::T7 gene1 [lon] ompT ahpC gal λatt::pNEB3-r1-cDsbC (SpecR, lacIq) ΔtrxB sulA11 R(mcr-73::miniTn10--TetS)2 [dcm] R(zgb-210::Tn10 --TetS) endA1 Δgor Δ(mcrC-mrr)114::IS10 | NEB cat# C3029 |
| MB2938 | C2566 + pZE1psoxS-GFP (AmpR) | This study |
| MB2940 | C3029 + pZE1psoxS-GFP (AmpR) | This study |
| MB2942 | C2566 + pZE1pdps-GFP (AmpR) | This study |
| MB2944 | C3029 + pZE1pdps-GFP (AmpR) | This study |
| MB4856 | C2566 + pQE60-Grx1-roGFP2-His (AmpR) | This study |
| MB4857 | C3029 + pQE60-Grx1-roGFP2-His (AmpR) | This study |
| MB4854 | C2566 + pQE60-roGFP2-Orp1-His (AmpR) | This study |
| MB4855 | C3029 + pQE60-roGFP2-Orp1-His (AmpR) | This study |
| MB5994 | C2566 + pQE60 (AmpR) | This study |
| MB5995 | C3029 + pQE60 (AmpR) | This study |
| Plasmids | Features | Source |
| pZE1psoxS-GFP | GFP under the regulation of soxS promoter, pBR322 origin, AmpR | [36] |
| pZE1pdps-GFP | GFP under the regulation of dps promoter, pBR322 origin, AmpR | [36] |
| pQE60-Grx-roGFP2-His | Grx1-roGFP2 fusion under the regulation of T5 promoter, pBR322 origin, AmpR | [21] |
| pQE60-roGFP2-Orp1-His | roGFP2-Orp1 fusion under the regulation of T5 promoter, pBR322 origin, AmpR | [21] |