Figure 1.

mRNA Delivery Can Overcome Current Limitations in Gene Therapy of Bone Marrow Cells

(A) Schematic representation of the hBM processing. (B) hBM stained using acridine orange dye; the images show control cells and cells exposed to complexed mRNA, respectively. The cells in red have higher amounts of mRNA. (C and D) Jurkat cells (C) and hBM cells (D) incubated with pDNA or mRNA, respectively, encoding for EGFP and complexed with lipidic complexing agents. (E and F) Quantification of EGFP-positive Jurkat cells (E) and hBM (F) shows that the delivery of complexed mRNA is more effective than pDNA. Asterisk represents statistically significant differences using paired Student’s t test (n = 4), p < 0.05.