Fig. 3.
(A) State 1 H2O2 production in mitochondria from permeabilized fibers from the TA muscles determined by oxidation of amplex red. (B) Total glutathione (GSH) and oxidized glutathione (GSSG) contents in TA muscles from control (non-denervated) mice and mice at 1, 3, 7, 14 and 21 days post-denervation. (C) A representative lane of an OxyBlot for control muscle showing the bands detected with approximate molecular weights (MW) indicated. (D) Quantification of protein carbonyls by densitometric analysis of the protein bands at different molecular weights. Histograms represent the mean and standard error of the mean for each experimental group (n = 4). *p < 0.05 - **p < 0.01 - ***p < 0.001 compared with the control group.