Fig. 4.

(A) State 1 mitochondrial H₂O₂ generation from permeabilized fibers of the TA muscle in the presence of different enzyme inhibitors. Addition of each inhibitor (chlorgyline (monoamine oxidase A), parglyline (monoamine oxidase B), apocynin (NADPH oxidase), AACOCF3 (PLA2) resulted in significantly reduced H₂O₂ generation compared to untreated fiber bundles at 14 and 21 days post-denervation. Histograms represent the mean and standard error of the mean for each experimental group (n = 4). *p < 0.05 - **p < 0.01 - ***p < 0.001 compared with its state 1. (B) Quantification of western blots of NADPH oxidase 2 subunits (Nox2/gp91^phox and p67^phox) and NADPH oxidase 4 (mitochondrial isoform) and (C) of cPLA2 and monoamine oxidase A content in TA muscle from control mice (non-denervated) and mice at 1, 3, 7, 14 and 21 days post-denervation. Histograms represent the mean protein content and standard error of the mean for each experimental group (n = 4). *p < 0.05 - **p < 0.01 - ***p < 0.001 compared with the control group.