Fig. 7.

PRDX1 expression and its link to breast cancer cellular response to AA. (A) PRDX1 mRNA expression patterns in log₂ values in 29 breast cancer cells retrieved from publicly available transcriptomic datasets of the Institut Curie breast cancer cell lines. Mean and median values are shown as solid and dashed lines, respectively. (B) Spearman's correlation analysis corresponding to PRDX1 mRNA expression vs IC50 for AA on 13 breast cancer cell lines. IC50 values were calculated from MTT assays for cell viability. Spearman's correlation was performed using GraphPad software. The 7 TNBC cell lines are indicated by green symbols and the 6 non-TNBC cell lines by purple symbols. The best-fit line is in solid orange line and the 95% confidence bands of the best-fit line are indicated in blue lines. Mathematical parameters are represented next to the graphs. (C) logIC50 of AA for breast cancer cell lines and their PRDX1 mRNA expression in log2 values. (D-E-F) MTT assay on HCC-1954, MDA-MB-436 and MDA-MB231 cells transiently transfected with PRDX1 specific siRNA or control siRNA for 48 h followed by treatments with different concentrations of AA for 24 h. The insert is Western blot to verify efficiency of PRDX1 knockdown in siRNA transfected cells. Statistical significance is assessed by two-way ANOVA with Sidak's multiple comparisons test. ****, P < 0.0001.