Figure 3.

Timing Analysis and Its Results for the Citric Acid Cycle

(A) Timing analysis. To determine the timing of the metabolic change upon H₂O₂ exposure, multivariate adaptive regression splines were fit to each temporal trace (log2[x vs 0 min H₂O₂]) of each metabolite.

(B) Timing analysis results for the citric acid cycle. Timing analysis results for all organisms and conditions considered, for the intermediates of the citric acid cycle. The half-time to local maximum t_1/2 was not determined (1) for spline fits with R2 < 0.2 and (2) if no significant maxima could be identified (i.e., peak prominence of Δlog2(FC) < 0.2). Furthermore, following local maxima with less than 50% change of log₂(FC) were removed.