Figure 2.

Immune characterisation of Zika virus (ZIKV)‐infected pregnant women. Blood samples were obtained from three pregnant women during the acute and convalescent phases of ZIKV infection. The women were infected in different trimesters of their pregnancy: P1, trimester 1; P2, trimester 2; and P3, trimester 3. Levels of peripheral blood immune cells and immune mediators were quantified by Luminex and immunophenotyping, respectively. Healthy controls (n = 3) were included. (a) Principal component analysis scores of the infected and healthy women based on a collective analysis of immunophenotyping and cytokine measurements with multiple factor analysis (MFA). (b) Correlation of immunophenotyping and cytokine measurements with the first two principal component (PC1 and PC2) axes of the MFA. (c) Heatmaps comparing the cytokine and immunophenotyping data for each subject. (d) Correlation between infection (% ZIKV NS3⁺ monocytes) and neutrophil numbers in whole blood samples.