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. 2019 Nov 5;15:1744806919881846. doi: 10.1177/1744806919881846

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© The Author(s) 2019

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Strategy to generate humanized chimeric NaV1.7 rat. (a) Rat Na_V1.7 exon 25 (blue vertical line) was replaced with the corresponding sequence in human Na_V1.7, which is human exon 26. (b) Schematic representation of the Human Na_V1.7 exon 26 sequence (blue shading) inserted into rat Na_V1.7 sequence (white shading) to generate the resulting chimeric Na_V1.7 channel. (c) Predicted topology of rat Na_V1.7 protein with insertion of human exon 26 coding sequence (blue shading) comprised of the first two transmembrane domains and S2-S3 intracellular loop of domain IV. (d) Representative restriction digest analysis of PCR products originating from genomic DNA from HOM-KI (A and B), HET (C) and WT (D) rats. (e) Open-field basic movement assessment. HOM-KI (5997 ± 220 counts) and WT (5318 ± 281 counts) rats had similar levels of exploratory movement (t₁₈ = 1.9, p = 0.07, unpaired t-test, n = 10/group). (f) Open-field rearing count assessment. HOM-KI (215 ± 7 counts) and WT (196 ± 10 counts) rats had similar rearing counts (t₁₈ = 1.6, p = 0.13, unpaired t-test, n = 10/group). (Data are mean ± SEM).