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. 2019 Oct 30;12:260. doi: 10.3389/fnmol.2019.00260

Figure 2.

Figure 2

Hippocampal neurons were fixed at DIV7 and immunostained against MAP-2 for neurites analysis, or fixed at DIV21 and immunostained against PSD95 or synaptophysin for synapse analysis. (A) Representative images of MAP-2 immunostaining on hippocampal neurons at DIV7. Scale bar, 20 μm. (B) Sholl analysis of hippocampal neurons demonstrated that loss of miR-137 significantly increased the number of intersection at 60–240 μm from the soma compared to non-treated neurons (n = 46–52 neurons from three to four mice, ***p < 0.001, two-way ANOVA). miR-137 cKO neurons showed increased dendritic length (C), dendritic ends (D) and number of nodes (branch points, E; n = 46–52 neurons from three to four mice, ***p < 0.001, two way ANOVA). (F) Cultured hippocampal neurons were fixed and stained for PSD-95. (G) Quantification of average PSD95 puncta intensity demonstrated that miR-137 cKO neurons had increased number of PSD95 puncta (n = 43 neurons per genotype; ***p < 0.001). Scale bar, 5 μm. (H) Cultured hippocampal neurons were fixed and stained for synaptophysin. (I) The average number of synaptophysin puncta was also increased in miR-137 cKO neurons (n = 40 neurons per genotype; ***p < 0.001). Scale bar, 5 μm.