Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 5;20(11):e47732. doi: 10.15252/embr.201947732

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 The Authors. Published under the terms of the CC BY 4.0 license

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

A
Summary of the effects of Gas2L1 shRNA‐mediated depletion, Gas2L1 overexpression (G2L1 OE) and low nanomolar doses of jasplakinolide (jasp) and Latrunculin B (LatB) on axon development of DIV3–4 neurons. o = no effect; ↓ = lower values, ↑ = higher values.

B
Proposed working model of the role of Gas2L1 during axon branching and outgrowth. MT binding by the tail of Gas2L1 promotes local actin binding and stabilization by the CH domain and shifts the actin‐stabilizing activity of Gas2L1 towards the axon. In growth cones, where dynamic MTs probe the actin‐rich periphery, Gas2L1 contributes to actin stability levels necessary for normal outgrowth. Reduced actin stability causes overextension of branches in the absence of Gas2L1. Axons of neurons overexpressing Gas2L1 have increased actin stability, resulting in shorter branches with higher F‐actin levels and buckling MTs.