Figure 6. Weight gain in GR^BATKO mice is comparable to Flox animals with prolonged high‐fat feeding.

• A
  Body weight in Flox and GR^BATKO after 24 weeks of high‐fat feeding (n = 10–13 animals per group).
• B
  BAT and iWAT weight in Flox and GR^BATKO mice fed HFD for 24 weeks (n = 10–13 animals per group).
• C
  GR levels in BAT after 24 weeks high‐fat feeding (n = 10–13 mice per group).
• D
  Representative H&E‐stained sections of BAT from Flox and GR^BATKO animals. Scale bar 100 μm.
• E
  Triglyceride content in BAT per gram of wet tissue (n = 10–13 animals per group).
• F
  qRT–PCR analysis of thermogenic marker gene expression in BAT of Flox and GR^BATKO mice after high‐fat feeding (n = 9–13 animals per group).
• G
  Representative immunohistochemistry for UCP1 in BAT. Scale bar 100 μm.
• H
  Serum insulin levels determined by ELISA (n = 10–13 animals per group).
• I
  Blood glucose in Flox and GRBATKO animals after 24 weeks HFD (n = 10–13 animals per group).

Data information: Data were analyzed with two‐tailed Student's t‐test (C, F). *P < 0.05; **P < 0.01. Error bars denote SEM.