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. 2019 Sep 23;20(11):e48150. doi: 10.15252/embr.201948150

Figure EV5. YAP1 subcellular localization (related to Fig 7).

Figure EV5

  1. A549 cells were transiently transfected with the indicated siRNA (control and KPT conditions = siNT) at low or high confluence. Forty‐eight hours later, cells were incubated overnight in the presence of XPO1 inhibitors KPT‐185 and KPT‐330 (final concentration = 1 μM) or with DMSO for all other conditions. The next day, cells were fixed and stained for endogenous YAP1. Representative images are shown, and scale bars are 40 μm (see Appendix Fig S7A for STK38 silencing).
  2. Genome‐edited XPO1 HAP1 cells were cultured for 2 days at low versus high confluency. Cells were then fixed and stained for endogenous YAP1. Representative images are shown, and scale bars are 40 μm.
  3. Genome‐edited XPO1 mutant HAP1 cells were transiently transfected with siRNA targeting endogenous STK38 (siSTK38#206 or with non‐targeting siRNA (siNT)) at low or high confluence. Seventy‐two hours later, cells were fixed and stained for endogenous YAP1. Representative images are shown, and scale bars are 40 μm (see Appendix Fig S7D for STK38 silencing).