Figure 3. Production of IL‐17A and IL‐17F is driven by STAT3 during inflammation but not at steady state.

Flow cytometric analysis of lymph node γδ T cells in RORγt^CRE‐STAT3^F/F (Cre⁺) and littermate control mice (Cre⁻). In graphs, each symbol represents a mouse or experiment and line the median. *P < 0.05, **P < 0.01, ***P < 0.001 using Mann–Whitney test (B, E, G, H) or 2‐way ANOVA (C, F).

• A–F
  (A, D) Representative dot plots showing IL‐17A (A) or IL‐17F (D) and IFNγ production in γδ T cells before (steady state) and after IMQ‐induced psoriasis. Numbers in gate indicate % positive cells; numbers outside the gate indicate mean fluorescence intensity of IL‐17A or IL‐17F. (B, E) Frequency of IL‐17A⁺ (B) and IL‐17F⁺ (E) γδ T cells before (steady state) and after IMQ‐induced psoriasis. (C, F) Quantification of mean fluorescence intensity (MFI) of IL‐17A (C) and IL‐17F (F) staining in γδ T cells after IMQ‐induced psoriasis (each color represents a different experiment). In (A‐C) steady state: n = 8; 4 experiments, IMQ: n = 11–12; 4 experiments. In (D‐F) steady state: n = 6; 3 experiments, IMQ: n = 8; 3 experiments. In (C) ***P < 0.001 with 2‐way ANOVA, in (F) *P < 0.05 with Mann‐Whitney or ***P < 0.001 with 2‐way ANOVA.
• G, H
  Frequency of IL‐17A⁺ (G) and IL‐17F⁺ γδ T (H) cells or IL‐17A (G) and IL‐17F (H) MFI following culture with IL‐1β, IL‐23, or nothing. Each symbol represents one experiment. Open circles = Cre⁺ (each color represents a different culture condition).