Skip to main content
. 2019 Sep 16;20(11):e47957. doi: 10.15252/embr.201947957

Figure EV3. Gene expression profiles in skeletal muscles.

Figure EV3

  1. Skeletal muscles of the lower legs were dissected from R3hdml KO (n = 5) and wild‐type control (n = 9) mice. Known myogenic factors were evaluated by real‐time PCR. Closed columns, wild‐type controls; open columns, R3hdml KO mice. Data are expressed as the means ± standard error of the mean (SEM). *P < 0.05; **P < 0.01; Student's t‐test.
  2. Total RNA was extracted from neonatal skeletal muscles for RNA sequencing, followed by gene set enrichment analysis for the KEGG‐derived cell cycle pathway in R3hdml KO and wild‐type control mice. FC W/KO, fold change WT/R3hdml KO. Normal P‐value 0.00, FDR q‐value 0.00.
  3. RNA sequencing results evaluated by real‐time PCR. Mki67, a marker for proliferation; Cdkn1a, a marker for inhibition of proliferation; E2f1 and Ccnd1, markers important in the G1 to S transition. Closed columns, wild‐type controls; open columns, R3hdml KO mice. Data are expressed as the means ± standard error of the mean (SEM). *P < 0.05; **P < 0.01; ***P < 0.001; Student's t‐test.