FIG 1.

Generation of C. muridarum transposon mutants. (A) Schematic of the pCMC5M plasmid used for transformation, containing the pUC ori, Himar1 C9 transposase, and a transposon with cat-gfp and flanking inverted repeat (IR) sequences. (B) Overview of procedure for generating C. muridarum transposon mutants in McCoy cells. (C) Genome map of transposon insertions in the C. muridarum chromosome and plasmid pNigg. Insertions in coding sequences (CDS) are in red; insertions in intergenic regions (IGR) are in gray. Nucleotide markers on C. muridarum chromosome are expressed in kilobase pairs.