Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Stability of transposon inserts. Two plaque-purified transposon mutants, UWCM012 (pmpA::Tn) and UWCM027 (glgB::Tn), were grown continuously for 10 generations in the presence (+) or absence (−) of 0.5 μg/ml chloramphenicol (cam). Genomic DNA from the passaged strains was extracted and subjected to PCR analysis using primers designed against genomic sequences flanking the transposon inserts. The ∼2-kb bands denote the sizes of transposon inserts.
