FIG 4.

Deficient glycogen accumulation in glgB::Tn inclusions. McCoy cells were infected with WT C. muridarum, a glgB::Tn mutant, a plasmid-free strain of C. muridarum (Cm P–), or a complemented glgB::Tn strain expressing WT glgB. Iodine staining of the infected cells at 26 hpi showed glycogen accumulation in WT and complemented inclusions (dark orange), no glycogen in Cm P– inclusions, and amylose accumulation in glgB::Tn inclusions (purple with dark deposits). Representative inclusions in each image are marked with asterisks.