TABLE 1.

Chlamydia muridarum transposon mutants

Straina	Insert position (TA location)b	Locus	Gene	Protein	% predicted translated
UWCM001	242824>25	TC0206		Hypothetical protein	86
UWCM002	785559<60	TC0657	pgi	Glucose-6-phosphate isomerase	30
UWCM003	472705>06	TC0411		Inc (predicted)	99
UWCM004a	35408>09	TC0031	gyrA2	DNA topoisomerase IV subunit A	67
UWCM004b	730561>62	TC0610	uvrA	Excinuclease ABC subunit A	11
UWCM005	1018583	TC0878		Hypothetical protein	11
UWCM006	818430<31	IGR
UWCM007a	301598>99	TC0257	glgB	14-Alpha-glucan-branching protein	86
UWCM007b	498439>40	TC0431	macP	MAC/perforin family protein	8
UWCM008	303619>20	TC0259		Hypothetical protein	90
UWCM009	220664>65	TC0189		Hypothetical protein	77
UWCM010	363126>27	TC0305		Hypothetical protein	80
UWCM011	687198<99	TC0575	pknD	Serine/threonine protein kinase	7
UWCM012	827362<63	TC0693	pmpA	Outer membrane protein PmpA	77
UWCM013	413657<58	TC0350	folD	Bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/cyclohydrolase	25
UWCM014a	232911>12	TC0197	pmpD	Outer membrane protein PmpD	67
UWCM014b	395719<20	TC0333	gnd	6-Phosphogluconate dehydrogenase	98
UWCM015	503579<80	TC0435		Phospholipase	66
UWCM016	505694>95	IGR
UWCM017	887618<19	TC0473		Acetyltransferase	66
UWCM018	1065355<56	IGR
UWCM019	319531<32	TC0267	pmpI	Outer membrane protein PmpI	70
UWCM020	94501>02	IGR
UWCM021	26638<39	IGR
UWCM022	361528<	TC0303		Hypothetical protein	0
	363601c	TC0306		Hypothetical protein	70d
UWCM023a	524741>42	TC0438		Cytotoxin/adherence factor	86
UWCM023b	1001585<86	IGR
UWCM024	429854<55	TC0368	ribF	Bifunctional riboflavin kinase/FMN adenyltransferase	2
UWCM024		TC0369	truB	tRNA pseudouridine synthase B	98
UWCM025	726016>17	TC0606		Exodeoxyribonuclease 7 small subunit	32
UWCM026	251258<59	TC0212	rmuC	DNA recombination protein RmuC homolog	60
UWCM027	301598>99	TC0257	glgB	1,4-Alpha-glucan branching protein	86
UWCM028	1006942<42	IGR
UWCM029	4770>71e	CDS6		pNigg CDS6	64
UWCM030	641212<13	TC0530		Protein phosphatase	72
UWCM031	358972<73	TC0302	recD	ATPase AAA	86
UWCM032	959708<09	TC0828	mip	Peptidyl-prolyl cis-trans isomerase	15
UWCM033	87859<60	TC0075		Hypothetical protein	22

^aa and b indicate two separate transposon insertions.

^bGenome location on C. muridarum chromosome (NC_002620); > and < indicate the orientation of the gfp-cat transposon in the genome.

^cInsert occurred with a 2,072-bp deletion beginning at 361529; genes TC0304 and TC0305 were deleted.

^dTruncation due to transposon insertion occurred at the 3′ end of TC0306.

^eGenome location on C. muridarum plasmid pMoPn (NC_002182).