FIG 2.

C. burnetii Dot/Icm substrates display three profiles of IcmS dependency. THP-l cells were infected for 48 h with wild-type C. burnetii or the icmS mutant (ΔicmS) producing the indicated Dot/Icm substrates fused to CyaA. Histograms depict the fold change in cytosolic [cAMP] relative to cells infected with C. burnetii producing CyaA alone (CyaA). (A) Ten substrates were secreted only by wild-type C. burnetii (IcmS dependent); (B) 29 substrates were secreted by both wild-type C. burnetii and the ΔicmS mutant (IcmS independent); (C) 11 substrates were secreted only by the ΔicmS mutant (IcmS inhibited). The cutoff for positive secretion is indicated by a dotted line at 2.5-fold change [cAMP]. Error bars indicate the standard deviations from the means for two independent experiments conducted in duplicate. Asterisks indicate substrates with significantly different translocation efficiencies by wild-type versus ΔicmS bacteria (P < 0.01, Student t test).