FIG 5.

Exchange of C-terminal secretion signals alters IcmS dependency. Histograms depict the fold change in cytosolic [cAMP] for THP-l cells infected for 48 h with wild-type C. burnetii or the icmS mutant producing the indicated CyaA fusion proteins. (A) Secretion of CBU0072 and CBU1525 fused to the C-terminal 50 amino acids of CBU0794 (CBU0072 ΔCT:CBU0794 or CBU1525 ΔCT:CBU0794, respectively). (B) Secretion of CBU0794 fused to the C-terminal 50 amino acids of CBU0072 or CBU1525 (CBU0794 ΔCT:CBU0072 or CBU0794 ΔCT:CBU1525, respectively). The cutoff for positive secretion is indicated by a dotted line at 2.5-fold change [cAMP]. Error bars indicate the standard deviations from the means for two independent experiments conducted in duplicate. The asterisk indicates a statistically significant difference (P < 0.01).