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IcmSW binds IcmS-dependent and -inhibited substrates. Immunoblots of GST-tagged IcmW and XpressT-tagged CBU0077, CBU0794, CBU066, or CBU1525 with or without their C-terminal 50 amino acids. GST-IcmW, IcmS, and individual substrates were produced together by IVTT. After incubation of cell extracts with GST-affinity beads, unbound (Flow through) and bead-bound (GST pulldown) proteins were immunoblotted with antibodies against the GST or XpressT tags.
