TABLE 1.
Dot/Icm substrates tested in this studya
| ORF | Name(s) | Size (kDa) | Translocation results (reference[s])b |
|---|---|---|---|
| CBU0021 | CvpB, Cig2 | 93.1 | Lp CyaA (28), Cb CyaA (47) |
| CBU0041 | CirA | 82.7 | Lp BlaM [80] (48) |
| CBU0072 | AnkA | 45.5 | Lp CyaA (WT < IcmS) (31), Lp CyaA (49) |
| CBU0077 | MceA | 29.6 | Lp CyaA, Cb BlaM [29] (3), Cb BlaM 38% (8) |
| CBU0080 | 18.7 | Lp CyaA (3), Cb BlaM [1] (6) | |
| CBU0129 | 11.1 | Lp BlaM 70% (48) | |
| CBU0145 | AnkB | 38.5 | Lp CyaA (WT > IcmS) (31), Lp CyaA (49) |
| CBU0295 | 56.8 | Lp CyaA (3), Lp BlaM [2] (6) | |
| CBU0329 | 23.3 | Lp CyaA (3) | |
| CBU0410 | 63.3 | Lp BlaM [45] (48) | |
| CBU0414 | 30.4 | Lp BlaM [45] (48) | |
| CBU0425 | CirB | 51.9 | Lp CyaA (3) |
| CBU0447 | AnkF | 21.3 | Lp CyaA (WT = IcmS) (31), Lp CyaA (49) |
| CBU0534 | 46.1 | Cb CyaA (47) | |
| CBU0635 | 55.3 | Lp CyaA, Cb BlaM [7.7] (3) | |
| CBU0665 | CvpA | 38.0 | Cb CyaA (47) |
| CBU0781 | AnkG | 38.6 | Lp CyaA (WT = IcmS) (31), Lp CyaA (49) |
| CBU0794 | 53.1 | Lp BlaM [80] (48) | |
| CBU0885 | 44.9 | Lp CyaA (28), Lp BlaM (6), Cb CyaA (47) | |
| CBU1024 | AnkH | 88.1 | Lp CyaA (WT > IcmS) (31), Lp CyaA (49) |
| CBU1045 | 40.0 | Lp BlaM [40] (48) | |
| CBU1107 | 35.5 | Lp CyaA (3) | |
| CBU1108 | 21.8 | Lp CyaA (3) | |
| CBU1217 | 57.8 | Lp BlaM [10] (48), Cb CyaA (47) | |
| CBU1460 | 29.6 | Lp BlaM [40] (48) | |
| CBU1461 | 67.1 | Lp BlaM [2] (48) | |
| CBU1493 | 64.2 | Cb CyaA (47) | |
| CBU1524 | CaeA | 25.1 | Lp CyaA (3), Cb BlaM [10], Cb BlaM [38] (8) |
| CBU1525 | 43.9 | Lp CyaA (3) | |
| CBU1532 | CaeB | 16.5 | Lp CyaA (3) |
| CBU1556 | CvpC | 64.6 | Lp BlaM [50] (48), Cb CyaA (47) |
| CBU1569 | 63.1 | Lp BlaM [90] (48) | |
| CBU1599 | 12.5 | Lp BlaM [10] (48) | |
| CBU1614 | 16.2 | Cb CyaA (46) | |
| CBU1676 | 41.8 | Lp CyaA (15), Cb CyaA (47) | |
| CBU1751 | Cig57 | 48.8 | Lp BlaM [60] (48) |
| CBU1776 | 24.0 | Lp CyaA (3) | |
| CBU1790 | 73.8 | Cb CyaA (46) | |
| CBU1818 | CvpD | 53.9 | Lp CyaA (15), Cb CyaA (47) |
| CBU1819 | 42.3 | Cb CyaA (47) | |
| CBU1823 | IcaA | 40.3 | Lp CyaA, Cb BlaM [10] (3), Cb BlaM [13] (8) |
| CBU1825 | 13.3 | Lp CyaA (3) | |
| CBU1863 | CvpE | 66.2 | Cb CyaA (47) |
| CBU1963 | 72.9 | Lp CyaA (3) | |
| CBU2052 | CirD | 34.0 | Lp CyaA (3) |
| CBU2056 | 25.4 | Lp CyaA (3) | |
| CBUA0013 | CpeB | 28.0 | Lp CyaA (30), Cb CyaA and BlaM (30) |
| CBUA0014 | CpeC | 8.7 | Lp CyaA (30), Cb CyaA and BlaM (30) |
| CBUA0015 | CpeD | 26.3 | Lp CyaA (30), Cb CyaA and BlaM (30) |
| CBUA0016 | CpeE | 39.5 | Lp CyaA (30), Cb CyaA and BlaM (30) |
a
C. burnetii Nine Mile (RSA 493) open reading frames (ORFs) of proteins tested for secretion by the C. burnetii Dot/Icm T4BSS are indicated, along with names and predicted molecular weights.
b
Translocation results with beta-lactamase (BlaM) and adenylate cyclase (CyaA) reporter assays in L. pneumophila (Lp) or C. burnetii (Cb) are indicated for the referenced studies. Where reported, the percentage of BlaM-positive cells is indicated in brackets, as well as the relative secretion efficiency in wild-type L. pneumophila (WT) versus the icmS mutant (IcmS).