FIG 7.

Open reading frame map of the plasticity zones for parental strains C. trachomatis L2tet9, C. muridarum transposon mutant CM007, and three PZ-based recombinants strains (KU2043, RC768, and RC215). C. muridarum ORFs are indicated with solid colors, while C. trachomatis ORFs are indicated with hatched colors. Homologous ORFs between strains are shown in black, green, orange, and blue. Plasticity zone genes unique to each species are shown in light gray, and the C. muridarum tox loci (truncated) are yellow. The transposed sequence within macP (TC0431) contributed by the CM007 parent is indicated in red. The 32-bp recombination target (ter) is indicated with a bullseye symbol. Homologous recombination at this target sequence forms a crossover point in each of the PZ-containing recombinant strains. Gene numbers are indicated by the species of origin.