FIG 8.

The 32-bp recombination target within the chlamydial PZ is conserved among strains and forms the chlamydial replication termination sequence. (A) BLAST analysis of the 32-bp recombination target among Chlamydia spp., Chlamydia-like organisms, and E. coli. The genomic sources of these data are indicated in Table S3 in the supplemental material. (B) Neighbor-joining phylogenetic tree built with the data from panel A. The scale bar represents the number of substitutions per site. (C) GCskew analysis of the C. trachomatis L2/434 chromosome showing an inflection point in the data at the position of the 32-bp recombination target (bullseye symbol), supporting the conclusion that this is the chlamydial replication terminator. Selected loci are indicated for reference purposes beneath a genome position scale (in kilobases).