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. 2019 Sep 10;20(11):e47016. doi: 10.15252/embr.201847016

Figure 6. Mutational analysis on the relevance of DB12 pseudoknots in ZIKV replication.

Figure 6

  • A, B
    Mutational schemes of the two PK3 mutants, ZIKV‐PK3^ (A) and ZIKV‐PK3* (B), mapping to the secondary structures of the DB12 region.
  • C
    E protein expression of ZIKV and the mutants showed by IFA.
  • D
    The progeny virus RNA in cultured supernatants of ZIKV and the mutants was detected by qRT–PCR in three technical replicates. Two‐way ANOVA and Dunnett's multiple comparison test were used for statistical analysis. The error bars represent the standard deviation. ***P < 0.001, ****P < 0.0001.
  • E
    The luciferase activity of the ZIKV‐Rep and the mutants was measured at different time points after transfection in three technical replicates. The error bars represent the standard deviation.